Order of Draw Part II, Serum Separator Tubes

order of draw lab requisition and tubes

Laboratory_Req_2016_Front


Gold-Top Serum Separator Tube (SST®): This tube contains a clot activator and serum gel separator – used for various chemistry, serology, and immunology tests. If the specimen requirement for a test is red-top tube(s), do not use gold-top/SST® tube(s).

NOTE: Invert the tube to activate the clotting; let stand for 20-30 minutes before centrifuging for 10 minutes. If frozen serum is required, pour off serum into plastic vial and freeze. Do not freeze Vacutainer® tubes.

Red-Top Tube, Plastic: This tube is a plastic Vacutainers containing a clot activator but no anticoagulants, preservatives, or separator material. It is used for collection of serum for selected laboratory tests as indicated. If the specimen requirement for a test is red-top tube(s), do not use gold-top/SST® tube(s), as the gel separator may interfere with analysis.

Red-Top Tube, Glass: This tube is a plain glass Vacutainer® containing no clot activators, anticoagulants, preservatives or separator material. These tubes can be used for Blood Bank tests and waste tubes when using a butterfly, except if doing blood cultures using aerobic and anaerobic vials. (with these types of blood culture vials, oxygen must be put into the vial from the ambient air in the butterfly tube, therefore there would not be any waste tubes used)

Light Green-Top Tube (lithium heparin): This tube contains lithium heparin and gel separator– used for the collection of heparinized plasma for routine chemistry tests.

NOTE: After the tube has been filled with blood, immediately invert the tube 8-10 times to mix and ensure adequate anticoagulation of the specimen.

DARK GREEN TOP TUBE FOR STAT DRAWS

This tube contains sodium heparin or lithium heparin – used for the collection of heparinized plasma or whole blood for special tests.

NOTE: After the tube has been filled with blood, immediately invert the tube 8-10 times to mix and ensure adequate anticoagulation of the specimen.

Test Description Tube Type

(Electrolyte Panel (Lytes) Sodium, Potassium, Chloride, CO2 )

SST Tube

Basic Metabolic Panel (BMP)

Electrolyte Panel, BUN, Calcium, Creatinine, Glucose SST Tube

Comprehensive Metabolic Panel (CMP)

Electrolyte Panel, BUN, Calcium, Creatinine, Glucose, Albumin, Alk. Phos., ALT (SGPT), Total Protein, AST (SGOT), Total Bilirubin, SST Tube
Lipid Panel (LIPID)

Cholesterol, HDL Cholesterol, Triglycerides, LDL (calculated) SST Tube
Hepatic Function Panel (HFP)

Albumin, Alk. Phos., AST (SGOT), ALT (SGPT), Direct Bilirubin, Total Bilirubin, Total Protein
SST Tube

Renal Panel (RENAL)

Electrolyte Panel, Albumin, BUN, Creatinine, Calcium, Glucose, Phosphorus
SST Tube

Prenatal Panel (PRENP)

CBC(Reflex), ABO & Rh (Blood Type), Antibody Screen (Reflex), RPR (Reflex),, Rubella, Hepatitis B. Surface Antigen (Reflex)
2 SST Tubes

Acute Hepatitis Panel (HPABC)

Hepatitis A Antibody IgM, Hepatitis B Surface Antigen (Reflex), Hepatitis B Core Antibody IgM, Hepatitis C Antibody
SST Tube

ORDER OF DRAW FOR SST”S

GOLD, THEN RED, THEN GREEN (REMEMBER Green is stat, but you must still use order of draw. Do not draw first!)